Study Molecular and Immunological Techniques for Detection of Leishmania Tropical, Major in Baghdad , Iraq

The study was conducted on 444 patients suffering from skin lesions of different ages and genders that were diagnosed by dermatologists as cutaneous leishmaniasis for people reviewing throughout the days week department epidemiology transitional diseases who attended to hospitals in city Baghdad, namely (Al-Shaheed Dari Al Fayyad Teaching Hospital , Al-Shaheed Sadr Al-Numan Hospital, Al-Karama Hospital) period October 1, 2019 until end March 2020. methods molecular diagnostics showed multiplex pcr examined40 random samples parasite  collected throughthis technique blood taken ulcers infectedpeople reveal DNA parts Leishmania parasite. Specialprefixes parasite species 28 positive samplescontain parasite's 12 negative samples, which 11 are forthe tropica type (27.5%) 17 major type(42.5%) at weight 1150 base pair. 30 serum with serological tests. current a significant decrease immunoglobulin G 1205.100 ± 210.155 results also level M 90.366 26.181